Journal article
Microfluidic Examination of the "hard" Biomolecular Corona Formed on Engineered Particles in Different Biological Milieu
ACG Weiss, K Kempe, S Förster, F Caruso
Biomacromolecules | AMER CHEMICAL SOC | Published : 2018
Abstract
The formation of a biomolecular corona around engineered particles determines, in large part, their biological behavior in vitro and in vivo. To gain a fundamental understanding of how particle design and the biological milieu influence the formation of the "hard" biomolecular corona, we conduct a series of in vitro studies using microfluidics. This setup allows the generation of a dynamic incubation environment with precise control over the applied flow rate, stream orientation, and channel dimensions, thus allowing accurate control of the fluid flow and the shear applied to the proteins and particles. We used mesoporous silica particles, poly(2-methacryloyloxyethylphosphorylcholine) (PMPC)..
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Grants
Awarded by Seventh Framework Programme
Funding Acknowledgements
This research was conducted and funded by the Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology (Project No. CE140100036). This work was also supported by the ARC under the Australian Laureate Fellowship (F.C., FL120100030) and the European Research Council (ERC)-Advanced Grant STREAM (Project 291211). A.C.G.W. acknowledges funding from The University of Melbourne through Melbourne International Research Scholarships and the German Academic Exchange Service (DAAD) through its Thematic network Bayreuth-Melbourne Colloid/Polymer Network sponsored from funds of the Federal Ministry of Education and Research (BMBF). K.K. gratefully acknowledges the award of an NHMRC-ARC Dementia Research Development Fellowship (APP1109945). We acknowledge M. Schlenk for device fabrication and assistance with equipment, K. Kruger for data analysis and useful discussions, Dr. M. Hund for assistance with AFM imaging, and Dr. M. Fuszard for mass spectrometry measurements. We also thank staff at the Electron Microscopy Keylab facility of the University of Bayreuth, the Melbourne Advanced Microscopy Facility (MAMF), and the Melbourne Magnetic Resonance Facility (MMRF) for experimental support.